Manufacture of activated pancreatin and stabilization of same



DORA'ESTELLE NEUN, OF NEW YORK, N,

Nnw YORK, Nit.

Y., ASSIGNOR 'ro saw, cgummcx 00;, or A conronn'rron.

MANUFACTURE OF ACTIVATED PANCREATIN' AND STABILIZATION 01f SAME.

No Drawing.

To all whom it may concern:

Be it known that I, DoRA E. NEUN, a citizen of the United States, and a resident of the ,city of New York, borough of Manhattan, county and State of New York have invented new Improvements in the anufacture of Activated Pancreatin and Stabilization of Same, of which the following is a full, clear, and exact description.

This invention relates to means for preserving the amylolytic digestive power of activated pancreatin.

Pancreatin is ordinarily prepared from the pancreas of animals and contains several digestive enzymes, one of which, amylopsin,

p converts starch into soluble carbohydrates.-

Other'digestive ferments in the pancreatin emulsify and decompose the fats and convert the proteins into proteoses, peptones, and amino acids. As sold commerclally,

pancreatin is in the form of a dry powder and is tested for, its amylolytic power b the method described in the United States harmacopoeia, which briefly consists in the digesting of 7.5 grams of dry potato starch with 0.3 grams of pancreatin at 40 C. for

- exactly five minutes, at which time the di- -whether commercial articles oir made by gestion should have progressed to such an extent that iodine fails to reveal the presence of unconverted starchu It has been known that certainsubstances increase the digestive power of the amylopsin in the pancreatin, but only one has been used commercially, viz sodium chloride. The increase of power depends upon the proportion in which sodium chloride is used." For instance, a

mixture of about 85% pancreatin and 15% sodium chloride has about double the amylolytic ower of the pure pancreatin. However, have found that these mixtures,

myself, do not hold their strength, but I showed after a period of about seven months a'loss of from 33%,to 50% ofthe amyl'olytic power.

An object of this invention, therefore, is to add a substance to the pancreatin activated with sodium chloride, which will stabilize or preserve the amylolytic power of such a mixture.

Another object of this invention is to provide a substance for this purpose which does activityof the mixture.

sodium phosphate. A further-object of this invention is to Specification of letters Patent. .Patented Jan, 1'922 Application filed May 25,

1929. Serial No. 384,029.

provide a substance which may be taken into the human system without causing harmful substance, being a normal constituent of the blood; it will prevent the deterioration of the amylolytic power. of the pancreatinsodium chloride mixture, and will also increase lts amylolytic power above that normallycaused by the sodium chloride. However, inorder to be effective as a stabilizer the percentage of di-sodium phosphate in the mlxture must not be too small. I found that mixtures consisting of 79% to 80% pancreatln, from 10% to 13.5% sodium chloride, and from 7.5% to 10% di-sodium phosphate showed good keeping qualities, while a mixture containing only 1% di-sodium phosphate, 14% sodium chloride and 85% pancreatin did not keep itsamylolytic power:

It is thus seen that the addition of disodium phosphate in proper proportion will preserve the amylolytic power of the activated pancreatin so that it may be stored in tightly sealed containers for long periods of time without deterioration, and thus will be provided a commercial digestant which can be relied upon to give high and uniform amylolytic action.

The preparation of the improved activated pancreatin is as follows:

A properly defatted and finely ground chloride, andfinely grounddesiccated disodium phosphate are'mixed together until a uniform mixture is obtained. The preparation 7 tainers.

I would state in conclusion that while the described samples constitute a practical embodiment of my invention, I do not limit myself strictly to the details herein described, since manifestly the same can be'considerably varied without departure from the spirit of the invention as defined in the appended claims. Having thus described my invention, I claim as new and desire to secure by Letters Patent: 1

1. A composition of matter for use as'a digestant which contains 2. A composition of matter for useas a is kept in tightly sealed oo'npancreatin and didigestant which contains Eancr'eatin, sodium chloride-and di-sodium'p osphute.

3. A composition of matter for use as a digestant, which consists of approximately 5 7.5 to 10% di-sodiqm phosphate, approxlmately IO-13.5% sodium chloride and the balance of puncreati'n, allthe ihgredients being nfisture-free. I

composition of matter focus: as a DORA ESTELLE NEUN." 

